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Cell-free extracts Cell suspensions (1.5mL) were centrifuged for 5 min at 8,000 g and lysed by adding 0.5mL of lysis buffer (50mM Tris-Cl, 150mM NaCl, 50mM ethylenediamine tetraacetic acid [EDTA], pH 7.2), 50mM phenyl methyl sulfonyl fluoride (PMSF
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