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Indeed, that yeast requires large amounts of NAD + in the peroxisomal matrix for fatty acid β-oxidation and uses two strategies to achieve this requirement: 1) peroxisomal NADH is converted enzymatically to NAD + in situ , yielding two small metabolites (malate and glycerol-3-phosphate) that are exported to the cytosol, and 2) peroxisomal NADH is simply exchanged by cytosolic NAD +

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