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6 8 The working hypothesis, assembled across roughly a decade of work, is that overactive NNMT in fat cells lowers NAD+, blunts energy expenditure, and promotes fat storage — and that blocking it should raise adipocyte energy metabolism and reduce fat mass

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After excitation of terbium fluorescence at 337 nm, emission at 490 nm (terbium) and 520 nm (GFP) were recorded with a 70-μs delay over 600 μs to reduce background fluorescence, and the reaction was followed over 60 cycles of each data point using a PHERAstar FS microplate reader (BMG Labtech)