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Similarly, metabolic engineering of Escherichia coli Nissle 1917 (EcN-TRP), combined with double-layer microcapsule delivery, achieves colon targeting and elevates local levels of immunomodulatory indole metabolites (e.g., indole-3-propionic acid) (Li et al., 2024)
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if long-term storage of the reconstituted solution is necessary, aliquoting into single doses is recommended
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