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Erythrocytes in multiple sclerosis – forgotten contributors to the pathophysiology? - PMC
PCR was employed for screening using 2 primers (5’-TTGTCCCTCAGTCCAGTGTCGCCTG-3’, 5’-GCCAAGGGCCTGAAATCTGAGCTAC-3’) to generate a 434-bp wild-type allele product and a 545-bp 3xFlag-Bnip3 allele product under the following conditions: 95 °C for 3 min
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